Menu Close

What is chromatography and its type?

What is chromatography and its type?

Chromatography is an analytical technique used to separate mixture of chemical substances into its individual compounds. Different types of chromatography are used in lab. e.g. column chromatography, thin-layer chromatography, gas chromatography etc.

What does chromatography mean?

Chromatography is a process for separating components of a mixture. The different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another.

What is the different types of chromatography?

  • Types of chromatography. Column chromatography.
  • Column chromatography.
  • Ion- exchange chromatography.
  • Gel- permeation (molecular sieve) chromatography.
  • Affinity chromatography.
  • Paper chromatography.
  • Thin-layer chromatography.
  • Gas chromatography.

What is chromatography explain with diagram?

Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate.

What are the two main types of chromatography?

The two main types of chromatography are gas-liquid chromatography (GLC) and gas-solid chromatography (GSC).

What is the aim of chromatography?

The objective of chromatography is to separate the various substances that make up a mixture. The applications range from a simple verification of the purity of a given compound to the quantitative determination of the components of a mixture.

What is chromatography one word?

Chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase. (The term adsorption isotherm is often used when a solid phase is involved.)

What is chromatography with example?

An example of chromatography is when a chemical reaction is used to cause each of the different size molecules in a liquid compound to separate into their own parts on a piece of paper. …

How are different types of chromatography techniques different?

The chromatography systems differ based on the physical states of the phases used. Homogeneous techniques have both the stationary phase and the mobile phase as a liquid. Ex: Liquid-liquid chromatography. Heterogeneous techniques employ different stationary and mobile phases.

What is the principle and application of chromatography?

Chromatography- Principle, Types and Applications. Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.

Which is the compound mixture separated by chromatography?

Analyte/ sample is the compound mixture what needs to be separated by chromatography. Similar procedure used in all chromatography. Lets explain the process of column chromatography to understand the general procedure. A column is firstly packed with silica gel, the stationery phase. Then a solvent (mobile phase) is passed through the column.

What kind of solvent is used in column chromatography?

Column chromatography. This is also known as liquid column chromatography. In this type of chromatography silica or alumina is used as stationery phase and a suitable solvent is used as a mobile phase.