Contents
How do you make a Wrights stain?
Procedure
- Prepare a film of blood or bone marrow on a microscopic slide and allow to air dry.
- Place the air-dried smear on the slide staining rack, smear side facing upwards.
- Cover the blood film with undiluted staining solution.
- Let stand for 2-3 minutes.
- Add approximately equal amount of buffered water (pH 6.5).
What is Wrights stain made of?
Wright’s stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope.
How is Giemsa stain prepared?
The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
How do you make a 10% Giemsa stain?
Make up a 10% Giemsa solution with distilled/deionized water buffered to pH 7.2. If only one slide is to be stained, you will require about 3 ml of prepared stain. Allow 3 drops of stock Giemsa solution (from the Pasteur pipette) to each millilitre of buffered water to give a 10% solution.
What are the steps for Gram staining?
The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram’s Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with …
What is true of Giemsa staining?
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
What is the procedure for blood staining technique?
Use of Giemsa stain is the recommended and most reliable procedure for staining thick and thin blood films. Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue.
How to make 1 : 20 dilution of Giemsa stain?
To make 1:20 dilution of Giemsa stain add 2 ml of stock solution of Giemsa stain to 40 ml of phosphate buffer solution in a clean Coplin jar. You can also use the Distilled water instead of buffer but the results may vary. ⇒ Now, stain the Methanol fixed Blood smear with diluted Giemsa stain (1:20, v/v) for 20 min.
What are the components of Wright and Giemsa stain?
The components are oxidized eosin Y, methylene blue, and azure B. They stain the cytoplasm of cells an orange to pink color and nucleus a blue to purple. Wright and Giemsa stains are used to study blood cell morphology. More videos at Abnova http://www.abnova.com Loading…
What’s the best way to make Wright’s stain?
Mix by gentle blowing. A metallic sheen (or green ‘scum’) should appear on the slide if mixing is appropriate. Leave for 5 minutes. Without disturbing the slide, flood the distilled water and wash until the thinner parts of the film are pinkish red.
How to make thin blood smears with Giemsa?
1 Prepare fresh working Giemsa stain in a staining jar, according to the previous page. 2 Pour 40 ml of working Giemsa buffer into a second staining jar. 3 Place slides into the working Giemsa stain (2.5%) for 45-60 minutes. 4 Remove thin smear slides and rinse by dipping 3-4 times in the Giemsa buffer. 5 Dry the slides upright in a rack.