How do you find the radius of a centrifuge rotor?
If you are unable to obtain this information, the radius can be found by measuring in centimeters (cm) the distances between the center of the rotation and the bottom of the tube in the rotor.
What is the unit of centrifuge?
When dealing with centrifuges, you will come across two different units of measure: The Relative Centrifugal Force (RCF) and Revolutions Per Minute (RPM).
What is the formula of RCF?
Relative centrifugal force is the force acting on samples during centrifugation. It is expressed as multiples of the earth’s gravitational field (g). RCF, RPM and r are linked by the equation for calculating RCF. RCF = 11.2 × r (RPM/1000)2 or RCF = 1.12 × 10-5 (RPM)2.
Which way does a centrifuge spin?
It works by causing denser substances and particles to move outward in the radial direction. At the same time, objects that are less dense are displaced and move to the centre.
Is RPM and g the same?
g Force or Relative Centrifugal Force (RCF) is the amount of acceleration to be applied to the sample. A good, precise protocol for centrifugation instructs you to use the g force rather than RPMs because the rotor size might differ, and g force will be different while the revolutions per minute stay the same.
How is the radius of a centrifuge measured?
Note: To determine the radius of your centrifuge rotor, simply take the measurement from the center of the centrifuge rotor to the end of the test tube carrier. It is interesting to note that centrifugation speed and time may not be as critical in routine sample-handling procedures involving the use of benchtop microcentrifuge.
How to calculate the relative centrifugal force ( RCF )?
The formula to calculate the relative centrifugal force (RCF) can be written as: where r is the radius of the rotor (in centimeters), and RPM is the speed of the rotor in rotation per minute. Rotors in centrifuges are the motor devices that house the tubes with the samples.
Why does a centrifuge spin around a central axis?
As the rotor spins around a central axis, it generates a centrifugal force acting to move particles away from the axis of rotation. If the centrifugal force exceeds the buoyant forces of liquid media and the frictional force created by the particle, the particles will sediment.
How to calculate rpm of a centrifuge rotor?
How the calculator works. The calculator solves unknown values using the formula: g = (1.118 x 10 -5) x R x S 2. Where g is the relative centrifugal force, R is the radius of the rotor in centimetres and S is the speed of the rotor in revolutions per minute.