Contents
How do you find km from Lineweaver-Burk plot?
The Lineweaver-Burk Plot
- y = 1/V.
- x = 1/S.
- m = KM/Vmax
- b = 1/[S]
- x-intercept = -1/KM
How do you find Vmax and Km from a graph?
From the graph find the maximum velocity and half it i.e. Vmax/2. Draw a horizontal line from this point till you find the point on the graph that corresponds to it and read off the substrate concentration at that point. This will give the value of Km.
How do you calculate Km and Vmax?
v = Vmax – Km x v / [S]
- y intercept = Vmax.
- gradient = -Km.
- x intercept = Vmax / Km.
What is Vmax on Lineweaver-Burk plot?
Definitions for Interpreting Plot The dependent axis of the Lineweaver- Burk plot is the reciprocal of velocity. Vmax: maximum velocity of the reaction. The y-intercept of the Lineweaver- Burk plot is the reciprocal of maximum velocity.
How do you find Vmax?
So, knowing the initial rate, Vo, and the various concentration of the substrate, you can create a straight line. The line plot represents the slope of Km/Vmax and y-intercept of 1/Vmax. Next, use the reciprocal of the y-intercept to calculate the Vmax of the enzyme activity.
What are the units of KM and Vmax?
KM is a the concentration substrate required to approach the maximum reaction velocity – if [S]>>Km then Vo will be close to Vmax. KM is a concentration. It will have units of: (M),or ( M),etc. liter liter KM depends only on the structure of the enzyme and is independent of enzyme concentration.
How to find km on a Lineweaver Burk plot?
Ease of Calculating the Vmax in Lineweaver-Burk Plot Next, you will obtain the rate of enzyme activity as 1/Vo = Km/Vmax (1/ [S]) + 1/Vmax, where Vo is the initial rate, Km is the dissociation constant between the substrate and the enzyme, Vmax is the maximum rate, and S is the concentration of the substrate.
How are the Vmax and km of a line determined?
Appendix: Vmax and KM Can Be Determined by Double-Reciprocal Plots. A plot of 1/ V0 versus 1/[S], called a Lineweaver-Burk or double-reciprocal plot, yields a straight line with an intercept of 1/ Vmax and a slope of KM / Vmax ( Figure 8.36 ). The intercept on the x -axis is -1/ KM .
How is the Lineweaver Burk plot used in enzyme kinetics?
The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents −1/Km.
Lineweaver–Burk Plot. The plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934. Lineweaver–Burk Plot.