What is meant by a control plate What purpose does a control serve?

What is meant by a control plate What purpose does a control serve?

A control plate is a guide to interpreting the results of an experiment; typically the control maintains specified strains of bacteria with a known result pattern in any particular test. These plates are incubated under the same condition as the bacteria being tested.

What is the purpose of the LB plate?

Luria broth (LB) is a nutrient-rich media commonly used to culture bacteria in the lab. The addition of agar to LB results in the formation of a gel that bacteria can grow on, as they are unable to digest the agar but can gather nutrition from the LB within.

Which plates should be compared to determine if any genetic transformation has occurred?

Which plates should be compared to determine if any genetic transformation has occurred? Why? The LB/amp (-) pGLO and the LB/amp (+) pGLO plates should be directly compared. Cells which were not treated with DNA (-pGLO) should not be expressing the ampicillin resistance gene and will not grow on the LB/amp plates.

How can we determine if a bacterial cell has been genetically transformed?

If there are any genetically transformed bacterial cells they would be located on the +pGLO LB/amp and the +pGLO LB/amp/ara plates. This is because both these plates have the plasmid inside them which allows for the transformation. You just studied 16 terms!

Why is a control very important to have in the agar plate?

As a positive control, the last plate in the series should be an agar medium in which all strains tested should grow. This way you can confirm that cells were transferred to all secondary plates in the series. Otherwise, nutrients could be transferred between plates allowing cells to grow on an unfavorable medium.

What is a negative control plate?

A negative control is a test in which a change in the system is not predicted. In a bacterial transformation experiment, attempting to grow untransformed bacteria on an antibiotic plate is considered a negative control with respect to growth because we do not expect any growth.

Why does a bacterial lawn appear?

Bacterial lawn is a term used by microbiologists to describe the appearance of bacterial colonies when all the individual colonies on a Petri dish agar plate merge to form a field or mat of bacteria. Bacterial lawns find use in screens for antibiotic resistance and bacteriophage titering.

What is the purpose of the plate with just lb and pGLO bacteria?

Only the LB medium control plates allows this bacteria to grow (LB + amp) will kill the bacteria without the pGLO plasmid. Which plates should be compared to determine if any genetic transformation has occurred?

What purpose did the two control plates serve?

What purpose does a control plate serve? Answer: A contol plate is a point of comparison (guide) to help you interpret the experimental results. In this experiment, the control plates were both the –pGlo plates, for they did not have the plasmid containing the genes for green fluorescence and ampicillin resistance.

Why does arabinose make bacteria glow?

The bacterial genes that make digestive enzymes to break down arabinose for food are not expressed when arabinose is not in the environment. But when arabinose is present, these genes are turned on. In the presence of arabinose, the GFP gene is turned on, and the bacteria glow brilliant green when exposed to UV light.