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What is the pH range of bromocresol green?

What is the pH range of bromocresol green?

3.8-5.4

Indicator pH Range Alkali
5.0 Bromocresol green 3.8-5.4 blue
6.0 Bromocresol purple 5.2-6.8 purple
7.0 Bromophenol blue 6.0-7.6 blue
9.0 Chlorophenol red 4.8-6.7 red

What is the molar absorptivity of bromocresol green?

14300 L.mol
Molar absorptivity (ε) was 14300 L. mol-1. cm-1. Beer’s law was obeyed in the concentration range of 0.3929-3.929 and 0.3929-39.29 μg.

What technique is used to determine the pKa of bromocresol green?

Using spectrophotometric methods, the absorption characteristics of the acid and base form of Bromocresol green, an acid-base indicator, will be used to determine the acid dissociation constant for this indicator.

At what pH does bromocresol green change color?

Bromocresol green is used for this purpose because it exhibits a color change within the pH range of 3.8 to 5.4.

Is bromocresol green a good indicator?

It can be used in its free acid form (light brown solid), or as a sodium salt (dark green solid). It is also an inhibitor of the prostaglandin E2 transport protein (3). Intended Use: Bromo Cresol Green Indicator is recommended as a pH indicator in growth medium for microorganisms.

Is bromocresol green an acid or base?

Key Takeaways: Bromocresol Green Indicator Recipe Bromocresol green is a pH indicator that is yellow below pH 3.8 and blue over pH 5.4. Between pH 3.8 and 5.4 it is green. The indicator is made from bromocresol green powder dissolved in ethanol.

Is bromocresol green a weak acid?

The correct answer is C. In the titration of a weak acid with a strong base, the conjugate base of the weak acid will make the pH at the equivalence point greater than 7. Both methyl orange and bromocresol green change color in an acidic pH range, while phenolphtalein changes in a basic pH.

Why is bromocresol green blue?

Bromo Cresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). In aqueous solution, Bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance(1).

How is the absorbance of bromocresol green determined?

Absorbance of Bromocresol Green at Different pH Values pH 0.0 pH 14.0 pH 4.0 Buffer Solution pH 5.0 Buffer Solution 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 Wavelength (nm) Absorbance. I•Spec 20 part 3: Kaof bromocresol gr •Quinic acid in fruit juices, pages 4-6

What kind of dye is bromocresol green used for?

Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). It belongs to a class of dyes called sulfonephthaleins. It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations.

Which is the best form of bromocresol to use?

It can be used in its free acid form (light brown solid), or as a sodium salt (dark green solid). It is also an inhibitor of the prostaglandin E 2 transport protein. Additional applications include use in sol-gel matrices, the detection of ammonia, and the measurement of albumin in human plasma and serum.

How is bromocresol green used in TLC staining?

An ethanol solution (0.04 wt%) of bromocresol green has been proposed for TLC staining and is suitable for visualisation of the compounds with functional groups whose p Ka is below 5.0 (carboxylic acids, sulfonic acids, etc.). These appear as yellow spots on a light or dark blue background; no heating is necessary.