Contents
- 1 What are the common sources of technical errors that will result in ABO discrepancies?
- 2 What should be the first thing that a medical technologist can do when there is an ABO discrepancy?
- 3 What is an ABO discrepancy?
- 4 What is the principle of ABO blood grouping?
- 5 When to resolve group o Abo discrepancies?
- 6 What does an ABO typing discrepancy mean?
- 7 Why are there so many Abo discrepancies in India?
What are the common sources of technical errors that will result in ABO discrepancies?
Technical/Clerical errors leading to ABO discrepancy were noticed in 12 (9.3%) cases. The most frequent cause of ABO discrepancies in forward grouping was subgroups of A Antigen (44.6%) and in reverse grouping was cold autoantibody (23.9%). There were 11 (8.4%) cases with alloantibodies.
What should be the first thing that a medical technologist can do when there is an ABO discrepancy?
A few key tips to remember when encountering an ABO discrepancy:
- Retest the sample first, using a different method, if available.
- Check for technical or clerical errors.
- Remember that the weakest reactions are usually the ones that are in doubt.
- Complete the antibody screen and note positive reactions.
What is ABO discrepancy?
A generic term for a variety of situations in which the interpretation of a patient or donor’s ABO grouping results is unclear. ABO discrepancies happen for a wide variety of reasons, including technical errors, problems with red cell antigens, or problems with serum antibodies. …
What is an ABO discrepancy?
What is the principle of ABO blood grouping?
Principle: The ABO and Rh blood grouping system is based on agglutination reaction. When red blood cells carrying one or both the antigens are exposed to the corresponding antibodies they interact with each other to form visible agglutination or clumping.
What causes false positive in ABO grouping?
If red blood cell suspensions are too concentrated, they can give weaker results due to the increase in the antigen/antibody ratio. In addition, red blood cells may fail to completely migrate to the bottom of the microtube and could cause a false positive interpretation.
https://www.youtube.com/watch?v=ojh1gkffscg
When to resolve group o Abo discrepancies?
All discrepancies should be resolved before blood products are issued, unless there is an emergency.If transfusion is required before resolution, group O products should be supplied. These are not ABO discrepancies, but refer to a specific reaction grade with two distinct sets of reactions
What does an ABO typing discrepancy mean?
Despite all of the modern technologies and reagents available to blood bankers today, ABO discrepancies still occur. An ABO typing discrepancy means that the interpretations of the red cell (forward) testing do not agree with the serum (back) testing.
What should I do if my Abo is not correct?
In this situation, pre-warming of all reagents and blood samples used will resolve the problem. There are other antibodies that react at room temperature, which may bind with the cells used for back typing if these cells contain the corresponding antigen.
Why are there so many Abo discrepancies in India?
While a discrepancy does not appear at first, the problem surfaces when the patient’s serum is tested against O cells. Since anti-H is present they will agglutinate these cells. This is a very rare blood type to encounter; mainly found in India, it is the result of consanguineous marriage (both partners descending from the same ancestor).
https://www.youtube.com/watch?v=Q0wEB1z5u8E